pwzl neo myr flag ptk2 (Addgene inc)

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Addgene inc pwzl neo myr flag ptk2
Pwzl Neo Myr Flag Ptk2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pwzl+neo+myr+flag+ptk2/pWZL+Neo+Myr+Flag+PTK2+(Plasmid+%2320610)/pmc12637249-362-18-19
Average 88 stars, based on 6 article reviews

pwzl neo myr flag ptk2 - by Bioz Stars, 2026-11

88/100 stars

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Article Title: ADAR promotes lung adenocarcinoma migration and invasion through stabilization of FAK
Article Snippet: Puromycin (A1113803) and Geneticin (10131027) were purchased from Life Technologies.

Transduction:

Article Title: Obesity promotes urinary tract infection by disrupting urothelial immune defenses
Article Snippet: .. Retrovirus Transduction : FAK was overexpressed by retroviral transduction of cells with pWZL-Neo-Myr_Flag-DEST (empty control) or pWZL-Neo-Myr-Flag-PTK2 (Addgene, Watertown, MA, USA). .. Retrovirus was produced using Phoenix-AMPHO cells (ATCC, Manassas, VA, USA).

Retroviral:

Article Title: Obesity promotes urinary tract infection by disrupting bladder focal adhesion kinase signaling
Article Snippet: .. Retrovirus was produced by transfecting Phoenix-AMPHO cells (ATCC, Manassas, VA, USA) with retroviral plasmids pWZL-Neo-Myr-Flag-DEST (empty control) or pWZL-Neo-Myr-Flag-PTK2 (Addgene, Watertown, MA, USA) using the ProFection Mammalian Transfection System (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. Viral supernatant was collected 24 h post transfection, centrifuged, filtered through a sterile 0.45 μM PVDF membrane, supplemented with 8 μg/mL polybrene (Sigma-Aldrich, Burlington, MA, USA) and added directly to human urothelial cells cultured to 95% confluence in CnT-Prime Epithelial Proliferation Medium in 24 well plates.

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Plasmid Preparation:

Article Title: ADAR promotes lung adenocarcinoma migration and invasion through stabilization of FAK
Article Snippet: FAK intronic region chr8:141,702,243-chr8:141,703,155 (the intron 26) and the exon 27 was amplified from HBEC cDNA and cloned into pcDNA3.1 + (GenScript, FAK E+I WT ) and a T>C mutation at site chr8:141,702,274 ( FAK E+I edited) was generated by site-directed mutagenesis. .. The primer pairs used for mutations are listed in Table S . pWZL-Neo-Myr-Flag-PTK2 was a gift from William Hahn and Jean Zhao (Addgene plasmid #20610). .. Genomic DNA was extracted from LUAD cells using the Genomic DNA Purification Kit (ThermoFisher Scientific), in accordance with the manufacturer’s instructions.

Review